A Practical Guide to Implementing a BSL-2+ Biosafety Program in Laboratories

BSL-2+ is the commonly used term for laboratories where work with microorganisms is conducted in a BSL-2 laboratory with biosafety practices and procedures that are typically found at BSL-3. This hybrid approach has been in use for many years, however, most research institutions still find it challenging to decide when to use this approach and which BSL-3 practices to use, due to the fact that it’s not a recognized containment level. This paper outlines a practical approach for successfully implementing BSL-3 practices in a BSL-2 laboratory.